Welcome to Todos Santos!
Thank you for joining us for our workshop on wet-lab techniques in next-generation sequencing. Here is a summary of the topics that we are looking forward to covering.
Monday November 18
- 12:00-13:00 Lunch (optional upon request with additional cost)
- 13:00-13:30 Introduction to Todos Santos and Group Discussion
- 13:30-14:30 Illumina sequencing chemistry and adapters
- 14:30-15:30 DNA/RNA quality control: methods for estimation of nucleic acid concentration, purity and integrity
- 15:30-15:45 Introduction to 16S rRNA microbiome profiling
- 15:45-17:00 Wet-lab: Illumina 16S rRNA amplicon library construction
- 17:00-18:00 Evening reception
- 18:00-21:00 Dinner on your own
Tuesday November 19
- 07:00-07:45 Breakfast
- 08:00-08:30 Illumina Library quality control: Quantification
- 08:30-09:00 Illumina Library quality control: Size selection
- 09:00-10:30 Wet Lab: Magnetic bead cleanup and Illumina 16S amplicon library gel electrophoresis
- 10:30-11:00 Barcoding and multiplexing techniques
- 11:00-12:00 Fragmentation
- 12:00-13:00 Lunch
- 13:00-14:00 Introduction to long-read DNA sequencing technologies
- 14:00-16:30 Wet-lab: Construction of Oxford Nanopore libraries and sequencing on a MinIon
- 16:30-18:00 Excursion: Sea turtle release at the beach!
- 18:00-21:00 Dinner on your own
Wednesday November 20
- 07:00-07:45 Breakfast
- 08:00-08:30 Results from Oxford Nanopore sequencing run
- 08:30-09:00 Making choices in high-throughput sequencing projects
- 09:00-10:00 Introduction to RNA-seq methods
- 10:00-11:00 Experimental design in high-throughput sequencing projects
- 11:00-12:00 Flexible discussion time (UMIs, enrichment techniques, personal projects)
- 12:00-13:00 Lunch (optional upon request with additional cost)
Supported by Colorado State University and the National Science Foundation